Huvec Primary Cell Line

Promocell offers huvec isolated in standard endothelial cell growth medium.

Huvec primary cell line.

Preparing cell culture flasks for culturing huvec. Human umbilical vein endothelial cells huvec 10 donor pool. They are guaranteed to express cd31 105 and survive in culture through 15 population doublings except huvec xl tm guaranteed through 5 pd. Product overview lonza s cryopreserved huvecs are shipped as frozen primaries.

Keep the medium to surface area ratio at 1ml per 5 cm 2. Take the endothelial cell growth medium from the refrigerator decontaminate the bottle with 70 alcohol in a sterile hood. Human umbilical vein endothelial cells huvec provide a classic model system to study many aspects of endothelial function and disease such as normal abnormal and tumor associated angiogenesis oxidative stress hypoxia and inflammation related pathways in endothelia under normal and pathological conditions cardiovascular related complications associated with various diseases mode of. Lonza offers a comprehensive range of normal human primary endothelial cells and supporting media for cardiac aortic coronary iliac artery pulmonary artery cardiac microvascular.

Pipette 20 ml of endothelial cell growth medium to a t 75 flask. Passage normal huvec cells when culture has reached approximately 80 confluence. Performance testedat least 16 population doublings when cultured in medium 200 supplemented with low serum growth supplement lsgs guarant. Normal human umbilical vein endothelial cells huvec primary 309 09.

Primary human umbilical vein endothelial cells huvec are isolated from human umbilical vein and cryopreserved at the end of the primary culture the cells are. Passage normal huvec cells when culture has reached approximately 80 confluence. Normal human umbilical vein endothelial cells huvec primary 309 09. Warm complete growth medium to 37 c prior to use with the cells.

Human umbilical vein endothelial cells huvec 10 donor pool 233 08. Warm both the trypsin edta for primary cells atcc pcs 999 003 and the trypsin neutralizing solution atcc pcs 999 004 to room temperature prior to dissociation. Warm both the trypsin edta for primary cells atcc pcs 999 003 and the trypsin neutralizing solution atcc pcs 999 004 to room temperature prior to dissociation. All cells test negative for mycoplasma bacteria yeast and fungi.