Human Mesenchymal Stem Cell Culture Protocol

Using a human fibronectin 20 µg ml or a 0 1 gelatin coated 48 well tissue culture plate seed 20k cells well in 0 5 ml normal msc growth media scm015 or scm045 after an overnight incubation replace the culture media with osteomax xf differentiation media.

Human mesenchymal stem cell culture protocol.

Osteogenesis of mesenchymal stem cells. Cryopreserved poietics tm normal human bone marrow derived mesenchymal stem cells hmsc are guaranteed to have at least 0 75 million viable cells per vial differentiate down the adipogenic chondrogenic and osteogenic liniages when cultured in the recommended differentiation medium to express cd29 cd44 cd105 and cd166 and to not express. Seed mesenchymal stem cells. Media and cells we offer everything you need for human mesenchymal stem cell culture.

Our hmsc s are available from bone marrow adipose tissue and umbilical cord matrix of individual donors. Learn more about our hla. Mesenchymal stem cell protocols download a free copy of our gibco mesenchymal stem cell sourcebook for a complete product reference guide product recommendations applications data and publications to support your msc workflow. Coat the culture vessel.

Complete portfolio of primary human mesenchymal stem cells hmsc and optimized growth and differentiation media. Protocol for mesenchymal stem cell expansion mesenchymal stem cells mscs are currently widely used in clinical trials for cell therapy and regenerative medicine applications. Pre warm the completed stemxvivo msc expansion media in a 37 c water bath. Coat a 6well tissue culture plate with 10 μg ml human fibronectin or bovine fibronectin according to the instruction manual.

When handling biohazard materials such as human cells safe laboratory procedures should be followed and protective clothing should be worn. Culturing of mesenchymal stem cells note. This procedure uses 20 ml for each t75 flask used.